Fire synchronizes flowering and boosts reproduction in a widespread but declining prairie species.

Fire is an important determinant of habitat structure and biodiversity across ecosystems worldwide. In fire-dependent communities, similar to the North American prairie, fire suppression contributes to local plant extinctions. Yet the demographic mechanisms responsible for species loss have not been directly investigated. We conducted a 21-y longitudinal study of 778 individual plants of Echinacea angustifolia, a widespread perennial species with chronically limited mating opportunities, to explore how fire affects reproduction. In a large preserve, with management units on different burn schedules, we investigated Echinacea mating scenes, which quantify isolation from potential mates and overlap in the timing of flowering, to determine the extent to which fire influences the potential for sexual reproduction. We demonstrate that fire consistently increased mating opportunities by synchronizing reproductive effort. Each fire occurred during fall or spring and stimulated flowering in the subsequent summer, thus synchronizing reproduction among years and increasing the proximity of potential mates after a fire. Greater within-season flowering synchrony in postfire mating scenes further increased mating potential. The improved postfire mating scene enhanced reproduction by increasing pollination efficiency. Seed set in scenes postfire exceeded other scenes by 55%, and annual fecundity nearly doubled (88% increase). We predict the reproductive benefits of synchronized flowering after fire can alleviate mate-finding Allee effects, promote population growth, and forestall local extirpation in small populations of Echinacea and many other prairie species. Furthermore, the synchronization of flowering by burning may improve mating opportunities, reproduction, and the likelihood of persistence for many other plant species in fire-dependent habitats.

Mating Opportunity Increases with Synchrony of Flowering among Years More than Synchrony within Years in a Nonmasting Perennial.

The timing and synchrony of mating activity in a population may vary both within and among years. With the exception of masting species, in which reproductive activity fluctuates dramatically among years, mating synchrony is typically studied within years. However, opportunities to mate also vary among years in nonmasting iteroparous species. We demonstrate that studying only within-year flowering synchrony fails to accurately quantify variation in mating opportunity in an experimental population ([Formula: see text]) of a nonmasting species, Echinacea angustifolia. We quantified individuals' synchrony of flowering within and among years and partitioned the contribution of each measure to mean daily mating potential, the number of potential mates per individual per day, averaged over every day that it flowered during the 11-year study period. Individual within- and among-year synchrony displayed wide variation and were weakly correlated. In particular, among-year synchrony explained 39% more variation in mean daily mating potential than did within-year synchrony. Among-year synchrony could have underappreciated significance for mating dynamics in nonmasting species.

How functional traits, herbivory, and genetic diversity interact in Echinacea: implications for fragmented populations.

Habitat fragmentation produces small, spatially isolated populations that promote inbreeding. Remnant populations often contain inbred and outbred individuals, but it is unclear how inbreeding relative to outbreeding affects the expression of functional traits and biotic interactions such as herbivory. We measured a suite of 12 functional traits and herbivore damage on three genotypic cross types in the prairie forb, Echinacea angustifolia: inbred, and outbred crosses resulting from matings within and between remnant populations. Inbreeding significantly affected the expression of all 12 functional traits that influence resource capture. Inbred individuals had consistently lower photosynthetic rates, water use efficiencies, specific leaf areas, and had higher trichome numbers, percent C, and percent N than outbred individuals. However, herbivore damage did not differ significantly among the cross types and was not correlated with other leaf functional traits. Leaf architecture and low physiological rates of the inbred compared to outbred individuals imply poorer capture or use of resources. Inbred plants also had lower survival and fitness relative to outbred plants. Our results show that inbreeding, a phenomenon predicted and observed to occur in fragmented populations, influences key functional traits such as plant structure, physiology and elemental composition. Because of their likely role in fitness of individuals and ecological dynamics plant functional traits can serve as a bridge between evolution and community or ecosystem ecology.